| 张世阳,徐修才,焦莉莉,顾永昊,谈敏.根皮苷对糖基化低密度脂蛋白诱导大鼠视网膜微血管内皮细胞表达乳脂肪球表皮生长因子8的影响[J].中国临床保健杂志,2017,20(4):430-434. |
| 根皮苷对糖基化低密度脂蛋白诱导大鼠视网膜微血管内皮细胞表达乳脂肪球表皮生长因子8的影响 |
| Effects of phlorizin on the expression of MFG-E8 in rats retinal microvascular endothelial cells in response to glycated low density lipoproteins |
| 投稿时间：2016-11-16 |
| DOI：10.3969/J.issn.1672-6790.2017.04.029 |
| 中文关键词: 糖尿病视网膜病变 根皮苷 脂蛋白类,LDL 表皮生长因子 大鼠,Sprague-Dawley |
| 英文关键词: Diabetic retinopathy Phlorhizin Lipoproteins,LDL Epidermal growth factor Rats,sprague-dawley〖FL |
| 基金项目:安徽省自然科学基金(1408085MH208) |
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| 中文摘要: |
| 目的 研究根皮苷(PHL)对糖基化低密度脂蛋白(gly-LDL)诱导大鼠视网膜微血管内皮细胞(RMVEC)表达乳脂肪球表皮生长因子8(MFG-E8)的影响,探讨PHL治疗糖尿病视网膜病变(DR)的作用及其机制。方法 体外培养大鼠RMVEC,分别进行不同浓度的gly-LDL与联合PHL+gly-LDL的处理。采用TUNEL方法观察不同处理条件下大鼠RMVEC的细胞凋亡。利用蛋白质免疫印迹方法(Western blotting)检测大鼠RMVEC 表达MFG-E8的变化。结果 与对照组相比,gly-LDL诱导大鼠RMVEC发生明显的细胞凋亡形态学变化,凋亡细胞的比例显著增多达到36.52%(P<0.01),预先经PHL(800 μmmol/L)孵育后,细胞凋亡明显减少至20.45%(P<0.01)；与对照组(蛋白表达灰度值0.290±0.008)比较,gly-LDL诱导组蛋白MFG-E8表达明显降低,并呈现浓度依赖性(蛋白表达灰度值分别为0.215±0.009、0.165±0.002、0.117±0.014与0.092±0.004,P<0.01)；经PHL预处理后,gly-LDL诱导的MFG-E8表达明显升高,伴随PHL给药浓度的增加,MFG-E8的表达逐渐上调,差异有统计学意义(蛋白表达灰度值分别为0.343±0.008、0.373±0.002与0.404±0.006,P<0.01)。结论 PHL可能通过显著改善蛋白MFG-E8的表达,从而抑制gly-LDL诱导的大鼠RMVEC的细胞凋亡,实现对DR的保护作用。 |
| 英文摘要: |
| Objective To study the effects of phlorizin on the expression of MFG-E8 in rats retinal microvascular endothelial cells (RMVEC) in response to glycated low density lipoproteins (gly-LDL).Methods The primary RMVEC in rats were cultured and identified in vitro.The apoptosis of RMVEC induced gly-LDL was estimated using TUNEL.The expressions of MFG-E8 in RMVEC in the absence or presence of phlorizin were determined by Western blotting.Results Gly-LDL caused an obvious apoptosis of RMVEC compared with the control group (36.52% VS. 1.24%,P<0.01),while pretreatment with phlorizin attenuated gly-LDL mediated cells apoptosis significantly (20.45% VS. 36.52%,P<0.01).Moreover,stimulation of RMVEC with different concentrations of gly-LDL(0,12.50,25.00,0.00,0.00 μg/mL) resulted in a significant decrease in the expression of MFG-E8 (P<0.01),while the pretreatment of RMVEC with different concentrations of phlorizin (200,0,800 μmmol/L) significantly restored the gly-LDL induced decreased MFG-E8 levels in a dose dependent manner for 48h (P<0.01).Conclusions Phlorizin could inhibit RMVEC apoptosis in response to gly-LDL,possibly by the up-regulation of the protein MFG-E8 expression. |
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