文章摘要
裴志勇,张健,尹巧香,等.地黄低聚糖对过氧化氢-血清饥饿诱导的脂肪组织来源干细胞Bax/Bcl-2表达的影响[J].中国临床保健杂志,2016,(1):69-71.
地黄低聚糖对过氧化氢-血清饥饿诱导的脂肪组织来源干细胞Bax/Bcl-2表达的影响
Effect of Rehmannia glutinosa oligosaccharides on Hydrogen peroxide-and serum deprivation-induced apoptosis in miniswine adipose tissue-derived stem cells
投稿时间：2015-12-01  
DOI：10.3969/J.issn.1672-6790.2016.01.023
中文关键词: 干细胞移植  地黄  细胞凋亡  过氧化氢  Bcl-2相关X蛋白质  模型, 动物
英文关键词: Stem cell transplantation  Rehmannia glutinosa  Apoptosis  Hydrogen peroxide  Bcl-2-Associated protein  Models, Animal 〖FL
基金项目:国家高科技研究发展计划(2006AA02A105)
作者单位E-mail
裴志勇 北京军区总医院干部病房一科,北京 100700 peizy6618222@sina.com 
张健 北京军区总医院干部病房一科,北京 100700  
尹巧香 空军总医院干部病房心内科  
杨桂利 军事医学科学院基础医学研究所前沿交叉学科研究室  
赵玉生 解放军总医院老年心血管病研究所  
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中文摘要:
      目的 探讨地黄低聚糖(RGOs)对过氧化氢-血清饥饿诱导的小型猪脂肪组织来源干细胞(ASCs)Bax、Bcl-2表达的影响。方法 过氧化氢(200μM)联合血清饥饿(H2O2/SD,6 h)建立小型猪ASCs凋亡模型。RGOs(0.01 g/L、0.1 g/L、1 g/L、10 g/L)预处理12 h并继续干预6 h。Western-blot法测定细胞Bax、Bcl-2的表达。结果 RGOs在一定浓度范围(0.1 g/L～10 g/L)可以减轻H2O2/SD引起的ASCs损伤,表现在Bax表达下调,Bcl-2表达上调。结论 地黄低聚糖对过氧化氢-血清饥饿诱导的脂肪组织来源干细胞的凋亡具有保护作用。
英文摘要:
      Objective To investigate the effect of Rehmannia glutinosa oligosaccharides (RGOs) on hydrogen peroxide-and serum deprivation-induced apoptosis in adipose tissue-derived stem cells.Methods An apoptosis model in miniswine adipose tissue-derived stem cells (ASCs) was established by hydrogen peroxide-and serum deprivation-induced (H2O2/SD,6h)in vitro.RGOs (0.01g/L,0.1g/L,1g/L,10g/L) pretreated for 12 hours and continued presence for 6 hours.Expression of Bax and Bcl-2 was investigated by using Western blot analysis.Results RGOs significantly attenuated hydrogen peroxide-and serum deprivation-induced ASCs apoptosis,showing a down-regulated Bax expression and an up-regulated Bcl-2 expression with the concentrations between 0.1g/L-10g/L.Conclution Rehmannia glutinosa oligosaccharides can protect adipose tissue-derived stem cells against hydrogen peroxide-and serum deprivation-induced apoptosis.
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