| 张之龄,沈琪琦,朱健,童小文,徐兵,周吴刚.血必净对脂多糖诱导的急性肺损伤大鼠肺组织细胞间粘附分子-1表达的影响[J].中国临床保健杂志,2016,(4):400-408. |
| 血必净对脂多糖诱导的急性肺损伤大鼠肺组织细胞间粘附分子-1表达的影响 |
| Effect of Xuebijing on expression of ICAM-1 in the lung tissue of rats with acute lung injury induced by lipopolysaccharide |
| 投稿时间：2016-02-16 |
| DOI：10.3969/J.issn.1672-6790.2016.04.022 |
| 中文关键词: 急性肺损伤 脓毒症 胞间黏附分子1 大鼠,Wistar |
| 英文关键词: Acute Lung injury Sepsis Intercellular adhesion molecule-1 Rats,Wistar〖FL |
| 基金项目:全军“十二五”重点课题(BWS11J054) |
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| 中文摘要: |
| 目的 观察血必净对脂多糖诱导的急性肺损伤(ALI)大鼠细胞间粘附分子-1(ICAM-1)表达的影响。方法 将45只Wistar 大鼠采用随机数字表分为三组:(1)健康对照(Control)组(15只)、(2)脂多糖 (LPS)组(15只)、(3) 血必净(XBJ)组(15只)。LPS组和XBJ组采用股静脉注射LPS(5 mg/kg)制备大鼠ALI模型。造模前半小时开始给药,XBJ组给予血必净4 mL/kg；Control组和LPS组则给予等容积0.9%氯化钠注射溶液。在造模后6 h、12 h、24 h各随机处死大鼠5只,分别作为Ⅰ、Ⅱ、Ⅲ亚组,光镜下观察肺脏组织形态学变化,免疫组织化学法(IHC)检测肺组织中ICAM-1蛋白表达。结果 造模后LPS组随着实验时间的延长,肺组织ICAM-1蛋白呈逐渐增加趋势,至24 h达到高峰。与LPS组相比,血必净组各时间点肺组织ICAM-1蛋白表达明显降低(P＜O.05)。光镜显示LPS组肺组织病理形态学改变明显,而血必净组则明显减轻。结论 血必净可通过抑制脂多糖诱导的急性肺损伤大鼠肺组织增强的ICAM-1表达减轻脂多糖诱导的大鼠肺损伤程度。 |
| 英文摘要: |
| Objective To observe the effect of Xuebijing on expression of intercellular adhesion molecule-1(ICAM-1)in the lung tissue of rats with acute lung injury induced by lipopolysaccharide.Methods Forty-five Wistar rats were randomly divided into 3 groups by random number table method:(1)control group(n=15),(2)LPS group(n=15),(3) Xuebijing group(XBJ group)(n=15).Injection of LPS(5 mg/kg) into femoral vein to reproduce ALI model in LPS group and XBJ group.Male Wistar rats of XBJ group were given XBJ 4 mL/kg 30 min before the intravenous injection of LPS,whereas control group and LPS group were given equivalent volume normal saline.Five rats in each group were killed at 6 h,12 h and 24 h,which were divided into subgroup I,Ⅱ,Ⅲ.The pathomorphism changes of lung tissue were observed by light microscope,and expression of ICAM-1 in pulmonary was examined by immunohistochemistry.Results Expression of ICAM-1 were gradually increased with experimental time prolonged and reached the peak at 24h in LPS group after establishing model.ICAM-1 were significantly lower in XBJ group than LPS group at each time points (P<0.05).Compared to LPS group,the pathological change was significantly reduce in XBJ group under microscope.Conclusion Xuebijing can inhibit the expressions of ICAM-1 and reduce acute lung injury induced by lipopolysaccharide in the lung tissue of rats. |
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