| 季菲,李晓红,马蓉,等.Th2型细胞因子模式转换对高级别宫颈上皮内瘤样病变的影响[J].中国临床保健杂志,2019,22(3):331-336. |
| Th2型细胞因子模式转换对高级别宫颈上皮内瘤样病变的影响 |
| A shift to a peripheral T2-type cytokine pattern during the carcinogenesis of cervical cancer becomes manifest in CIN Ⅲ lesions |
| 投稿时间：2018-01-18 |
| DOI：10.3969/J.issn.1672-6790.2019.03.011 |
| 中文关键词: 宫颈肿瘤 宫颈上皮内瘤样病变 Th2细胞 白细胞介素类 肿瘤坏死因子α |
| 英文关键词: Uterine cervical neoplasms Cervical intraepithelial neoplasia Th2 Cells Interleukins Tumor necrosis factor-alpha 〖FL |
| 基金项目:国家自然科学基金(81660476) |
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| 中文摘要: |
| 目的 研究Th2型细胞因子变化对早期发现宫颈癌的诊断价值。 方法 研究对象为21例高危型人乳头瘤病毒(HPV)阳性的高级别宫颈上皮内瘤样病变(CIN Ⅱ-Ⅲ)的患者和12例浸润型宫颈癌患者。设立一个对照组为非CIN的10例HR-HPV阳性的患者,另一个对照组为11例HR-HPV阴性的女性。通过白细胞计数的差异来评估白细胞亚群的差异。用酶联免疫吸附法测定肿瘤坏死因子α(TNF-α),肿瘤坏死因子受体 (TNFR) Ⅰ和Ⅱ,干扰素γ(IFN-γ),白细胞介素2(IL-2),白细胞介素12(IL-12),白细胞介素4(IL-4),白细胞介素10(IL-10)的血浆浓度。 结果 CIN Ⅲ患者和宫颈癌患者的白细胞数目显著高于对照组,差异有统计学意义(P<0.05)。CIN Ⅲ患者和宫颈癌患者的血浆IFN-γ浓度显著低于CIN Ⅱ患者或者对照组(P<0.05),有统计学差异。IL-12,IL-2,IL-4和TNF-α的血浆浓度在组之间差别并不大(P>0.05),但在CIN Ⅲ患者和宫颈癌患者中,TNFRⅡ的血浆浓度明显低于CIN Ⅱ。在CIN Ⅲ患者和宫颈癌患者的血浆中可检测到高浓度的IL-10。 结论在宫颈癌发生过程中,CIN Ⅲ患者发生了向Th2型细胞因子模式的转移。 |
| 英文摘要: |
| Objective To study the diagnostic value of Th2 cytokine changes in early detection of cervical cancer.Methods 21 HR-HPV positive women with high grade cervical intraepithelial neoplasia (CIN Ⅱ-Ⅲ) and 12 patients with invasive cervical carcinoma were included in the study group.Two control groups consisted of 10 HR-HPV-positive or 11 HR-HPV-negative women without CIN,respectively.Differences in leucocyte subgroups were evaluated by a differential leucocyte count.Plasma concentrations of tumour necrosis factor a (TNF-α),TNF-α receptors TNFR Ⅰand TNFR Ⅱ,IFN-γ,interleukin 2 (IL-2),IL-12,IL-4 and IL-10 were determined by enzyme linked immunosorbent assays. Results Leucocyte counts in patients with CIN III and carcinoma were significantly higher than that in the controls(P<0.05).Plasma IFN-γ concentration was significantly lower in the patients with CIN III and carcinoma than that in women with CIN Ⅱ or controls (P<0.05).Plasma concentrations of IL-12,IL-2,IL-4 and TNF-α did not differ significantly between groups (P>0.05),but significantly lower plasma concentrations of TNFRⅡ were found in the patients with CIN Ⅲ and carcinoma compared with those with CIN Ⅱ.IL-10 was detected with increased frequency in the plasma of patients with CIN Ⅲ and carcinoma. Conclusion Patients with CIN Ⅲ have metastasized to Th2 cytokine pattern during cervical cancer. |
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