| 程蕤,姜辉,周晶,等.高效液相色谱法测定马西替坦中N,N-二甲基苯胺含量[J].中国临床保健杂志,2019,22(4):500-502. |
| 高效液相色谱法测定马西替坦中N,N-二甲基苯胺含量 |
| Determination of N,N-dimethylaniline in Macitentan by HPLC |
| 投稿时间：2018-07-10 |
| DOI：10.3969/J.issn.1672-6790.2019.04.018 |
| 中文关键词: 高血压,肺性 气相色谱-质谱法 内皮素受体拮抗剂 |
| 英文关键词: Hypertension,pulmonary Gas chromatography-mass spectrometry Endothelin receptor antagonists 〖FL |
| 基金项目:安徽省高等学校省级自然科学研究(重点)项目(KJ2015A0598) |
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| 中文摘要: |
| 目的 建立高效液相色谱法(HPLC)测定马西替坦中N,N-二甲基苯胺的含量。方法 采用高效液相色谱法,色谱柱为菲罗门 Luna C18色谱柱(250 mm×4.6 mm,5 μm),流动相为水(用磷酸调节pH值至2.5)∶乙腈=20∶80,检测波长254 nm,柱温30 ℃。结果 N,N-二甲基苯胺在0.031～0.937 μg/mL范围内线性关系良好(r=0.9995,n=5),平均回收率为99.58%,RSD为1.22%(n=9)。结论 HPLC可作为马西替坦中N,N-二甲苯胺含量测定方法。 |
| 英文摘要: |
| Objective To establish HPLC method for determining the content of N,N-dimethylaniline in Macitentan.Methods HPLC was performed on a Phenomenex Luna C18 column(250mm × 4.6mm,5 μm).The mobile phase is water(Adjust pH to 2.5 with phosphoric acid)- acetonitrile(20∶80).The detection wavelength is 254 nm,and the column temperature is 30 ℃.Results The linear of N,N-dimethylanilinewas in the rangefrom 0.031 to 0.937 μg/mL(r=0.9995,n=5).The average recovery was 99.58%,with RSD was 1.22%(n=9).Conclusions The HPLC can be used as a method for determining the content of N,N-dimethylaniline in Macitentan. |
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