文章摘要
书星,赵洋,孙敏,等.microRNA-21、PTEN、RASA1及caspase-3在肾上腺醛固酮瘤中的表达及相关作用机制[J].中国临床保健杂志,2021,24(6):826-830.
microRNA-21、PTEN、RASA1及caspase-3在肾上腺醛固酮瘤中的表达及相关作用机制
Expression and mechanism of microRNA-21,PTEN,RASA1 and caspase-3 in adrenal aldosteronoma
投稿时间：2021-06-10  
DOI：10.3969/J.issn.1672-6790.2021.06.023
中文关键词: 肾上腺醛固酮瘤  microRNA-21  PTEN  RASA1  HACC  caspase-3  荧光定量PCR  Western Blot  MTT  免疫组织化学
英文关键词: Adrenal aldosteronoma  microRNA-21  PTEN  RASA1  HACC  caspase-3  Real-time Quantitative PCR  Western Blot  MTT  Immunohistochemistry 〖FL
基金项目:新疆维吾尔自治区自然科学基金项目(2016D01C104)
作者单位E-mail
书星 新疆维吾尔自治区人民医院,泌尿中心,乌鲁木齐 830002 asd159213@126.commicrorna 
赵洋 新疆维吾尔自治区人民医院,科教中心,乌鲁木齐 830002 asd159213@126.commicrorna 
孙敏 新疆维吾尔自治区人民医院,泌尿中心,乌鲁木齐 830002 asd159213@126.commicrorna 
南玉奎 新疆维吾尔自治区人民医院,泌尿中心,乌鲁木齐 830002 asd159213@126.commicrorna 
王慧琴 新疆维吾尔自治区人民医院,科教中心,乌鲁木齐 830002 asd159213@126.commicrorna 
齐飞波 新疆维吾尔自治区人民医院,科教中心,乌鲁木齐 830002 asd159213@126.commicrorna 
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中文摘要:
      目的 研究microRNA-21(miR-21)、PTEN、RASA1及caspase-3在肾上腺醛固酮瘤中的表达情况,探究其内在相关性及对疾病的影响机制。方法 采用荧光定量PCR法和免疫组织化学法检测样本中miR-21及PTEN、RASA1、caspase-3的表达量,再用细胞转染建立人肾上腺皮质细胞(HACC)模型,采用Western Blot技术检测细胞模型中caspase-3表达量。结果 miR-21在肾上腺醛固酮瘤和瘤旁组织中的表达量分别为0.109±0.050和0.040±0.019,差异有统计学意义(P＜0.001)；其中瘤组织样本中PTEN阳性表达率为22.91%(P=0.001),瘤旁组织样本中PTEN阳性表达率85.42%(P=0.001),RASA1阳性表达率分别为8.33%和60.41%(P＜0.001),caspase-3阳性表达率分别为25.00%和89.58%(P＜0.001),均差异有统计学意义。HACC细胞模型显示,当miR-21过表达时,caspase-3蛋白表达下调,细胞表现出过度增殖趋势；miR-21表达抑制时,caspase-3蛋白表达上调,细胞表现为生长抑制。结论 肾上腺醛固瘤组织中miR-21高表达可能通过下调PTEN、RASA1及caspase-3,从而抑制细胞凋亡并参与肿瘤进展。
英文摘要:
      Objective To investigate expressions of microRNA-21 (miR-21),PTEN,RASA1 and caspase-3 in adrenal aldosteronoma and explore the internal correlation and the mechanism of its influence on the disease.MethodsThe expression of miR-21,PTEN,rasa1 and caspase-3 were detected by fluorescence quantitative PCR and immunohistochemistry respectively.HACC cell model was established by cell transfection,and caspase-3 expression was detected by Western Blot.Results The expression of miR-21 in adrenal aldosterone tumor and its adjacent tissues was 0.109±0.050 and 0.040±0.019,respectively (P<0.001).The positive expression rate of PTEN in tumor tissue and adjacent tissue was 22.91% and 85.42%,respectively (P=0.001).The positive expression rate of RASA1 in tumor tissue and adjacent tissue was 8.33% and 60.41%,respectively (P<0.001).The positive expression rate of caspase-3 in tumor tissue and adjacent tissue was 25.00% and 89.58%,respectively (P<0.001).The above data had significant differences.HACC cell model showed that when miR-21 was over expressed,caspase-3 protein expression was down regulated,and the cell showed an over proliferation trend;when miR-21 was down regulated,caspase-3 protein expression was down regulated,and the cell showed growth inhibition.Conclusion The high expression of miR-21 in adrenal aldehyde solid tumor tissue may be involved in tumor progression by down regulating PTEN,RASA1 and caspase-3.
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