| 张潇,彭楠,高月明,等.254 nm紫外发光二极管体外杀灭幽门螺杆菌量效关系研究[J].中国临床保健杂志,2025,28(2):239-243. |
| 254 nm紫外发光二极管体外杀灭幽门螺杆菌量效关系研究 |
| Study on the dose-effect relationship of killing of helicobacter pylori by 254 nm ultraviolet light-emitting diodes in vitro |
| 投稿时间：2024-12-02 |
| DOI：10.3969/J.issn.1672-6790.2025.02.018 |
| 中文关键词: 幽门螺杆菌 激光,半导体 紫外线 灭菌 |
| 英文关键词: Helicobacter pylori Lasers,semiconductor Ultraviolet rays Sterilization 〖FL |
| 基金项目: |
| 作者 | 单位 | E-mail | | 张潇 | 中国人民解放军医学院,北京 100853
中国人民解放军总医院第二医学中心,a保健二科, 康复医学科, 保健九科,d消化内科,北京 100853 | pengnan301@163.com254 | | 彭楠 | 中国人民解放军总医院第二医学中心,a保健二科, 康复医学科, 保健九科,d消化内科,北京 100853 | pengnan301@163.com254 | | 高月明 | 中国人民解放军总医院第二医学中心,a保健二科, 康复医学科, 保健九科,d消化内科,北京 100853 | pengnan301@163.com254 | | 王刚石 | 中国人民解放军总医院第二医学中心,a保健二科, 康复医学科, 保健九科,d消化内科,北京 100853 | pengnan301@163.com254 | | 陈红龙 | 中国人民解放军总医院第二医学中心,a保健二科, 康复医学科, 保健九科,d消化内科,北京 100853 | pengnan301@163.com254 | | 丁潇楠 | 中国人民解放军总医院第一医学中心肾脏病医学部 | pengnan301@163.com254 |
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| 中文摘要: |
| 目的 评估LED-254 nm短波紫外线(UVC)对幽门螺杆菌国际标准株SS1和临床分离耐药株C1的体外杀灭效果,分析比较暴露时间和辐照剂量对不同菌株杀灭效果的影响,建立量效关系模型。方法 将标准株SS1和耐药株C1体外培养后分别制备成浓度为1×106 CFU/mL的混悬液。将适量的幽门螺杆菌(H.pylori)混悬液滴入96孔板中,制备厚度为1 mm的菌液柱孔板,调整辐照距离为1 cm,接受不同时间(1、2、3、4、5、10、15 s)的LED-254 nm UVC照射。每组实验设置3个复孔,阴性对照组采用不开机的方式进行假照射。照射后,回收菌液,培养3 d,上述实验在相同条件下重复3次,通过平板菌落计数法计算杀灭率。结果 在标准条件下(菌液厚度1 mm,辐照距离1 cm),H.pylori的最小抑菌剂量(MID50)为4.89 mJ/cm2,完全杀灭阈值(MID99)为32.50 mJ/cm2,杀灭90%微生物总数的所需时间(D值)为1.62 s,杀灭时间(KT)为4.87 s。暴露时间、辐照剂量与杀灭效果均呈正相关,差异有统计学意义(Ptime<0.001,Pdose<0.001)。时效关系和量效关系可通过单相指数(one-phase exponential)模型精准拟合: ytime=100×[1-exp(-1.42×t)],R2=0.988 7；ydose=100×[1-exp(-0.142×d)],R2=0.998 7。标准株SS1和耐药株C1对于LED-254 nm UVC杀灭效能响应程度不具有差异性。结论 LED-254 nm UVC可应用于H.pylori的杀灭,在体外标准实验条件下展现出快速且高效的杀灭效能,并且LED-254 nm UVC对H.pylori的杀灭效能具有普适性,不受其菌株耐药性差异的干扰。时效关系模型和量效关系模型可用于预测不同时间、不同剂量下的杀灭效果,为后续实验提供了关键参数基准。 |
| 英文摘要: |
| Objective To evaluate the killing effect of LED-254 nm short-wave ultraviolet light (UVC) on the international standard strain Helicobacter pylori (H.pylori) SS1 and the clinical isolated drug-resistant strain H.pylori C1,analyze and compare the influence of exposure time and irradiation dose on the killing effect of different strains,clarify the important killing indicators and establish the dose-effect relationship model in vitro.Methods The standard strain H.pylori SS1 and the drug-resistant strain H.pylori C1 were cultured and then respectively prepared into suspensions with a concentration of 1×106 CFU/mL in vitro.An appropriate amount of H.pylori suspension was dropped into a 96-well plate to ensure that each well in it contains 1 mm thickness of bacterial suspension.The irradiation distance was adjusted to 1 cm,and LED-254 nm UVCirradiation was received for different times (1,2,3,4,5,10,15 s).Three replicate wells were set for each group of experiments,and the negative control group was subjected to sham irradiation by not turning on the machine.After irradiation,the bacterial liquid was recovered and cultured under suitable conditions for 3 days.The above experiments were repeated three times under the same conditions.The killing rate was calculated by the plate colony counting method.Results Under standard conditions (bacterial liquid thickness of 1 mm,irradiation distance of 1 cm),the MID50 of H.pylori was 4.89 mJ/cm2,the MID99 was 32.50 mJ/cm2,the D value was 1.62 s,and the KT value was 4.87 s.The exposure time,irradiation dose and killing effect were all significantly positively correlated,and the difference was statistically significant (Ptime<0.001,Pdose<0.001).The time-effect relationship and dose-effect relationship could be precisely fitted by the one-phase exponential model: ytime=100×[1-exp(-1.42×t)],R2=0.988 7；ydose=100×[1-exp(-0.142×d)],R2=0.998 7.The response degrees of the standard strain H.pylori SS1 and the drug-resistant strain H.pylori C1 to the killing efficacy of LED-254 nm UVC were not different.Conclusions LED-254 nm UVC can be applied to the killing of H.pylori,showing rapid and highly efficient killing efficacy under in vitro standard experimental conditions,and its physical inactivation characteristics are not affected by drug resistance genes.The time-effect relationship model and dose-effect relationship model can be used to predict the killing effect at different times and doses,providing key parameter benchmarks for subsequent experiments. |
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