| 王雨濛,彭文举,夏百荣.不可逆电穿孔对卵巢癌的效应及作用机制研究[J].中国临床保健杂志,2026,(1):127-132. |
| 不可逆电穿孔对卵巢癌的效应及作用机制研究 |
| Study on the effects and mechanisms of irreversible electroporation on ovarian cancer |
| 投稿时间：2025-12-17 |
| DOI：10.3969/J.issn.1672-6790.2026.01.022 |
| 中文关键词: 卵巢肿瘤 不可逆电穿孔治疗 细胞死亡 疾病模型,动物 |
| 英文关键词: Ovarian neoplasms Irreversible electroporation therapy Cell death Disease models,animal Fund program:National Natural Science Foundation of China |
| 基金项目:国家自然科学基金项目 |
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| 摘要点击次数: 50 |
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| 中文摘要: |
| 目的 探讨不可逆电穿孔(IRE)在体内外卵巢癌模型中,对卵巢癌细胞的杀伤效应及作用机制。方法 使用不同强度,脉宽及脉冲数对卵巢癌细胞系SK-OV-3进行处理,其中未处理的细胞作为对照,通过CCK-8及流式细胞术检测IRE对SK-OV-3细胞活性、凋亡及坏死的影响。使用Western bolt及qRT-PCR检测SK-OV-3凋亡相关蛋白表达,并对细胞活性氧水平、线粒体膜电位、腺嘌呤核苷三磷酸释放及细胞内钙离子(Ca2+)浓度进行检测,探究IRE处理后SK-OV-3细胞凋亡和坏死的机制。通过构建SK-OV-3皮下瘤模型,使用不同场强(0、400、800和1 000 V/cm)对皮下瘤进行处理,其中,0 V/cm处理组为对照组。观察IRE处理后,瘤体24 d体积变化及小鼠血清白细胞介素-1β、肿瘤坏死因子-α和干扰素-γ浓度测定,探究IRE对体内肿瘤生长的抑制作用及对小鼠抗肿瘤免疫的影响。结果 随着电场强度及脉冲数增加,对SK-OV-3细胞的杀伤效果增大,细胞坏死比例增加；电场强度达到800 V/cm时,活细胞比例:(7.14±0.16)%。动物皮下瘤模型显示,与对照组相比,IRE处理可显著限制小鼠皮下瘤生长；场强800 V/cm,脉宽100 μs条件下,50个脉冲可达到对SK-OV-3细胞最佳杀伤作用。结论 IRE可以通过破坏细胞内稳态,诱导SK-OV-3细胞死亡,作用于细胞的场强及脉冲数增加,细胞坏死比例也增加。 |
| 英文摘要: |
| Objective To explore the cytotoxic efficacy and underlying mechanisms of irreversible electroporation (IRE) on ovarian cancer cells in vitro and in vivo models.Methods SK-OV-3 ovarian cancer cells were treated with IRE under different electric field strengths,pulse widths,and pulse numbers,with untreated cells serving as the control.Cell viability,apoptosis,and necrosis were evaluated using the CCK-8 and flow cytometry.To investigate the mechanisms of IRE-induced apoptosis and necrosis in SK-OV-3 cells,the expression of apoptosis-associated proteins was examined using Western blot and qRT-PCR.Additionally,intracellular ROS levels,mitochondrial membrane potential,ATP release and Ca2+ concentration were assessed.By constructing a subcutaneous tumor model using SK-OV-3 cells and applying different electric field intensities (0,400,800 and 1 000 V/cm) to treat the tumors—with the 0 V/cm group serving as the control.A subcutaneous SK-OV-3 xenograft model was established to evaluate the inhibitory effect of IRE on tumor growth and its impact on anti-tumor immune response in vivo.Tumor volume was monitored for 24 d post-IRE treatment,and serum concentrations of IL-1β,TNF-α,and IFN-γ were measured to preliminarily assess the systemic immunodulatory effects.Results The cytotoxic effect on SK-OV-3 cells was enhanced with increasing electric field strength and pulse number,which was accompanied by a rise in the proportion of necrotic cells.When the electric field intensity reached 800 V/cm,almost no viable cells were detected [viable cell proportion:(7.14 ± 0.16)%].The subcutaneous tumor model in animals showed that,compared with the control group,IRE treatment significantly inhibits the growth of subcutaneous tumors in mice.Under conditions of 800 V/cm field strength and 100 μs pulse width,50 pulses achieve the optimal killing effect on SK-OV-3 cells.Conclusions IRE can induce SK-OV-3 cell death by perturbing intracellular homeostasis.The proportion of necrotic cells increases with higher electric field strength and pulse number. |
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