| 洪芳德,宫孟琦,赵家鑫,等.急性冠脉综合征患者血浆替格瑞洛及其代谢物浓度测定方法的建立与验证[J].中国临床保健杂志,2026,29(2):247-253. |
| 急性冠脉综合征患者血浆替格瑞洛及其代谢物浓度测定方法的建立与验证 |
| Establishment and validation of a method for the determination of ticagrelor and its metabolites in plasma of patients with acute coronary syndrome |
| 投稿时间：2026-04-06 |
| DOI：10.3969/J.issn.1672-6790.2026.02.020 |
| 中文关键词: 急性冠状动脉综合征 替格瑞洛 色谱法,液相 血药浓度 药物相关性副作用和不良反应 |
| 英文关键词: Acute coronary syndrome Ticagrelor Chromatography,liquid Ticagrelor Plasma concentration Drug-related side effects and adverse reactions Fund programs:National Natural Science Foundation of China |
| 基金项目:国家自然科学基金项目 |
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| 摘要点击次数: 20 |
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| 中文摘要: |
| 目的 建立同时测定人血浆替格瑞洛及其主要活性代谢物AR-C124910XX浓度的高效液相色谱法用于临床。方法 以甲醇为蛋白沉淀剂,流动相乙腈-水(50∶50),色谱柱安捷伦XB-C18(4.6 mm×250 mm,5 μm),检测波长299 nm,流速1.0 mL/min,柱温30 ℃,进样量20 μL。选取2022年11月至2026年3月在安徽医科大学第一附属医院使用替格瑞洛治疗的42例患者,测定并分析血药浓度。14例患者出现呼吸困难,纳入呼吸困难组；余28例,纳入对照组。结果 替格瑞洛和AR-C124910XX色谱峰分离良好,在100~4 000 μg/L范围内线性关系良好。高效液相色谱法的专属性、精密度、准确度、提取回收率、稳定性等均符合生物样本分析的相关要求。呼吸困难组人群血替格瑞洛浓度高于对照组,但两组活性代谢物浓度差异无统计学意义。结论 高效液相色谱法简便、快速、成本低、灵敏度高,能同时满足人血浆替格瑞洛及活性代谢物血药浓度监测要求,用其监测替格瑞洛血药浓度有助于预防其导致的呼吸困难。 |
| 英文摘要: |
| Objective To establish method for measuring plasma levels of ticagrelor and its main active metabolite AR-C124910XX by using high-performance liquid chromatographic,and apply it in the determination of clinical blood drug concentration.Methods Methanol was used as the protein precipitant,with the mobile phase being acetonitrile-water (50∶50);the chromatographic column was an Agilent XB-C18 (4.6 mm×250 mm,5 μm);the detection wavelength was set at 299 nm;the flow rate was 1.0 mL·min-1,the column temperature was maintained at 30 ℃ and the injection volume was 20 μL.A total of 42 patients who received ticagrelor treatment were recruited at the First Affiliated Hospital of Anhui Medical University from November 2022 to March 2026.14 patients displayed ticagrelor-induced dyspnea,another 28 patients were recruited as the control group.Results The chromatographic peaks of ticagrelor and AR-C124910XX were well separated.Measurement results of ticagrelor and AR-C124910XX showed a good linear relationship in the range of 100-4 000 μg/L.The specificity,precision,accuracy,extraction recovery rate and stability of this method all meet the relevant requirements for biological sample analysis.The concentration of ticagrelor in the dyspnea group was higher than that in the control group,while there was no statistically significant difference in the concentration of active metabolites.Conclusions The HPLC that we developed is simple,rapid,low cost,high sensitive,and monitoring the plasma concentration of ticagrelor by this method should be benificial for preventing ticagrelor-induced dyspnea. |
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