| 王心淼,郜青叶,金子夜,马大光,焦雪琴,狄旭.糖尿病性白内障晶状体上皮细胞的Fas、P53基因表达[J].中国临床保健杂志,2016,(1):37-39. |
| 糖尿病性白内障晶状体上皮细胞的Fas、P53基因表达 |
| Diabetic cataracts cataract lens epithelial cell apoptosis and the expression of Fas,P53 gene |
| 投稿时间：2015-06-11 |
| DOI：10.3969/J.issn.1672-6790.2016.01.012 |
| 中文关键词: 糖尿病并发症/白内障 上皮细胞/晶体 凋亡诱导因子 基因,p53 |
| 英文关键词: Diabetes complications/cataract Epithelial cells/lens,crystalline Apoptosis inducing factor Genes,p53〖FL |
| 基金项目:内蒙古科技厅2011年度自治区应用研发资金计划(20110501) |
|
| 摘要点击次数: 6728 |
| 全文下载次数: 0 |
| 中文摘要: |
| 目的 探讨糖尿病性白内障及老年性白内障晶状体上皮细胞(LEC)的凋亡与Fas、P53基因的关系。方法 在白内障超声乳化手术中取糖尿病性及老年性白内障患者的晶状体前囊膜标本45例,应用光镜,电镜观察LEC的凋亡情况,应用RT-PCR定量检测Fas蛋白,P53蛋白在白内障LEC中的表达。结果 两种白内障LEC中均有Fas,P53蛋白表达。Fas蛋白在糖尿病性白内障LEC中的表达平均是老年性白内障的13.34倍,P53蛋白在糖尿病性白内障LEC中的表达平均是老年性白内障的7.3倍,两者差异有统计学意义(P＜0.05)。结论 两种白内障LEC均出现凋亡,Fas、P53基因在糖尿病性白内障LEC凋亡中起重要作用。 |
| 英文摘要: |
| Objective To investigate the relationship between lens epithelial cells (LEC) apoptosis of the diabetic cataract and senile cataract and Fas,P53 gene.Methods Anterior lens capsules specimens 45 cases were took from diabetic and senile cataract patients in the cataract ultrasonic emulsification operation,using light and electron microscopic to observing apoptosis of LEC,and applying RT-PCR to detect quantitatively expression of Fas protein and Bax protein in cataract LEC.Results Two kinds of cataract in the LEC had both protein expression of Fas and P53.Fas protein expression in diabetic cataract LEC was 13.34 times in senile cataract.P53 protein expression in diabetic cataract LEC was 7.3 times in senile cataract,there were significant differences (P<0.05).Conclusion Fas and P53 genes may play an important role in cataract LEC apoptosis. |
|
查看全文
|
| 关闭 |
|
|
|