| 孙艳,沈静,单煜恒,钟士江.基质金属蛋白酶-2在脑胶质瘤细胞中的表达及其分子探针体外磁共振成像的检测[J].中国临床保健杂志,2018,21(1):88-91. |
| 基质金属蛋白酶-2在脑胶质瘤细胞中的表达及其分子探针体外磁共振成像的检测 |
| Expression of MMP2 in brain glioma cells and detection of magnetic resonance imaging in vitro by molecular probes |
| 投稿时间：2017-05-10 |
| DOI：10.3969/J.issn.1672-6790.2018.01.025 |
| 中文关键词: 神经胶质瘤 基质金属蛋白酶2 磁共振成像 分子探针 |
| 英文关键词: Glioma Matrix metalloproteinase 2 Magnetic resonance imaging Molecular probes 〖FL |
| 基金项目:中国人民武装警察部队后勤学院附属医院种子基金(FYM201535)；天津市卫生行业重点攻关项目(16KG136) |
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| 中文摘要: |
| 目的 观察基质金属蛋白酶-2(MMP2)在脑胶质瘤细胞中的表达,并检测其分子探针体外磁共振成像能力。方法 利用免疫印迹法检测MMP2在多种脑胶质瘤细胞系的表达水平；利用碳二亚胺法合成USPIO-PEG-MMP2Ab分子探针,进行噻唑蓝比色法检测其细胞毒性,普鲁士蓝染色及体外磁共振成像检测USPIO-PEG及USPIO-PEG-MMP2Ab与MMP2高表达的脑胶质瘤细胞的特异性结合能力。结果 所有胶质瘤细胞均高表达MMP2,与星形胶质细胞相比,差异有统计学意义；合成的分子探针USPIO-PEG-MMP2Ab应用浓度范围内无明显细胞毒性,具有同C6脑胶质瘤细胞特异性结合的能力,普鲁士蓝染色结果显示在相同浓度及时间的条件下,USPIO-PEG-MMP2Ab组较USPIO-PEG组蓝染颗粒更多、更明显；磁共振成像结果显示USPIO-PEG-MMP2Ab组细胞在相同浓度条件下SI值下降水平明显较高,与USPIO-PEG组细胞相比,差异有统计学意义。结论 MMP2在胶质瘤细胞中高表达,且USPIO-PEG-MMP2Ab分子探针活性高,安全性好,对脑胶质瘤细胞系具有靶向性。 |
| 英文摘要: |
| Objective Observation of matrix metalloproteinase -2 (MMP2) expression in the glioma cells,and study its molecular probes magnetic resonance imaging ability in vitro.Method The expression level of MMP2 was detected by Western blotting in various brain glioma cell line;USPIO-PEG-MMP2Ab was synthetized by conjugating USPIO-PEG with MMP2Ab.MTT colorimetric assay was used to assess the cytotoxicity of USPIO-PEG-MMP2Ab.The specific binding ability of USPIO-PEG and USPIO-PEG-MMP2Ab with MMP2 highly expressed glioma cells was detected by Prussian blue staining and MR imaging in vitro.Result All the tumor cells were positive for MMP2,there were significant differences compared with astrocytes;The synthetic molecular probe USPIO-PEG-MMP2Ab has no cytotoxicity in the range of concentration,and has the ability to bind specifically to C6 glioma cells.Prussian blue staining showed that at the same time and concentration conditions,USPIO-PEG-MMP2Ab group showed much more intracellular blue particles than USPIO-PEG group.Magnetic resonance imaging showed that the SI value of the group USPIO-PEG-MMP2Ab cells under the same concentration condition decreased significantly,and compared with the USPIO-PEG group cells,the difference was statistically significant.Conclusion MMP2 is highly expressed in glioma cells,and the USPIO-PEG-MMP2Ab molecular probe has high activity and safety.It is targeted against glioma cell lines and lays the foundation in vivo experiments. |
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