| 曹云祥,刘健,黄传兵,张皖东,汪元.雷公藤多苷通过抑制炎性因子及调整细胞基质改善佐剂性关节炎大鼠心功能的研究[J].中国临床保健杂志,2020,23(1):104-108. |
| 雷公藤多苷通过抑制炎性因子及调整细胞基质改善佐剂性关节炎大鼠心功能的研究 |
| Tripterygium improve cardiac function of AA rats by inhibiting inflammatory factors and adjusting cell matrix |
| 投稿时间：2019-12-23 |
| DOI：10.3969/J.issn.1672-6790.2020.01.024 |
| 中文关键词: 关节炎,实验性 心肌疾病 细胞基质 雷公藤属 模型, 动物 |
| 英文关键词: Arthritis,experimental Cardiomyopathies Cell-matrix Tripterygium Models,animal |
| 基金项目:国家自然科学基金青年基金项目(81503558)；国家重点研发计划项目(2018YFC1705204);安徽省高校优秀青年人才支持计划项目(gxyq2018028)；安徽省自然科学基金面上项目(1808085MH303) |
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| 中文摘要: |
| 目的 探讨雷公藤多苷(TPT)改善佐剂性关节炎(AA)大鼠心功能的机制。方法 皮内注射弗氏完全佐剂(FCA)复制AA大鼠模型,以TPT0.9%氯化钠注射溶液混悬液(每毫升含TPT1 mg),按1 mL/100 g的剂量灌胃治疗,自制容积测定仪测量各组大鼠右后足容积并计算关节炎指数(AI)；有创血流动力学检查AA大鼠心功能,免疫组织化学检测基质金属蛋白酶9(MMP-9)的蛋白表达情况；ELISA法测定血清细胞炎性因子白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)等变化；透射电镜观察大鼠心肌细胞超微结构改变。结果 TPT治疗后,AA大鼠的足趾肿胀度、关节炎指数水平下降,体质量升高(P＜0.05)；心脏指数(CI)、左室收缩期压(LVSP)、左室舒张期末压(LVEDP)水平下降,左室内压上升下降最大速率(±dp/ dtmax)水平提高(P<0.01)；IL-10显著升高(P＜0.01),TNF-α、IL-1β、MMP-9显著降低(P＜0.01)。结论 雷公藤多苷通过抑制炎性因子及调整细胞基质改善AA大鼠心功能。 |
| 英文摘要: |
| Objective To investigate the mechanism of tripterygium(TPT) to improve cardiac function of AA rats. Methods Intradermal injection of FCA replicable AA rat model,TPT saline suspension(each mL containing TPT 1 mg),administered at a dose of 1 mL/100 g gastric administration,use self-made volumetric instrument to measure the right posterior foot volume of each group of rats and calculate the arthritis index(AI).The cardiac function of AA rats was examined by hemodynamics; immunohistochemistry to detect protein expression of matrix metalloproteinase 9(MMP-9).The changes of serum inflammatory factors IL-10,TNF-α,IL-1 β were determined by ELISA.The ultrastructural changes of myocardial cells in rats were observed by transmission electron microscope. Results The level of toe swelling,arthritis index and weight of AA rats decreased after TPT treatment(P<0.05).The levels of LVEDP,LVSP,CI decreased,and the level of±DP / dtmax increased(P<0.01).IL-10 increased while TNF-α,IL-1 β,and MMP-9 decreased significantly(P<0.01). Conclusion Tripterygium polyglycoside can improve cardiac function in AA rats by inhibiting inflammatory factors and adjusting cell matrix. |
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